NucleoSpin® RNA Plus

RNA isolation / purification Tissue - Mouse Retina

Experiment
RNA isolation / purification Tissue - Mouse Retina
Product
NucleoSpin® RNA Plus from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- This kit is designed for tissue samples of < 30 mg tissue.
Downstream tips
- For adsorption measurement, use 5 mM Tris pH 8.5 as diluent.

Publication protocol

Total RNA was isolated from 1 × 105 BV2 cells or from individual mouse retinal extracts immediately after euthanasia using NucleoSpin® RNA Plus kit (Macherey-Nagel GmbH (available online: http://www.mn-net.com/)), following the manufacturers protocol.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Retina using NucleoSpin® RNA Plus from Macherey Nagel.

Paper title
Concentrated Conditioned Media from Adipose Tissue Derived Mesenchymal Stem Cells Mitigates Visual Deficits and Retinal Inflammation Following Mild Traumatic Brain Injury
Full paper
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA Plus below.

Download PDF Download manufacturer protocol

Videos

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